| Gene | Sc4mol |
| Chromosome | 8 |
| Deletion Start | 64,727,875 |
| Deletion End | 64,718,962 |
| Deletion Size | 8,914 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,727,819 | 64,727,838 | CCCTGGCTGTGGAGTATGTG |
| TUP | 64,727,791 | 64,727,815 | TCACTGTTGCCGGAGAATCCTCTGC |
| TUR | 64,727,724 | 64,727,745 | CAGGTTGCGATCTGGAACTTTG |
| TDF | 64,719,872 | 64,719,891 | CCCAAGAGTGTGCTGAAACC |
| TDP | 64,719,850 | 64,719,871 | TGGAGCCTCTGCCATGTTAGGC |
| TDR | 64,719,824 | 64,719,844 | CCAGCTCCACCCAGAGACAAG |
PCR Primers
| Primer | Sequence |
| SU | AAGCCTAATGCTTGCTTGATG |
| SD | TTCAGTCACTCGCTCACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 115 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17494A-D1 | VGB6 | C57BL/6NTac | |
| 17494A-B12 | VGB6 | C57BL/6NTac | |
| 17494A-F12 | VGB6 | C57BL/6NTac | |
| 17494A-D11 | VGB6 | C57BL/6NTac | |
| 17494A-B2 | VGB6 | C57BL/6NTac | |
| 17494A-G1 | VGB6 | C57BL/6NTac | |
