| Gene | Scyl3 |
| Chromosome | 1 |
| Deletion Start | 163,941,214 |
| Deletion End | 163,933,532 |
| Deletion Size | 7,683 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 163,933,995 | 163,934,018 | CCCTTAAAGTCACTGCTGCATTTG |
| TUP | 163,934,031 | 163,934,060 | AGTGCAGTCAGTTTGCTATTTGGTTCCATG |
| TUR | 163,934,093 | 163,934,117 | GACTGAGACATATCATCAGCTCAAG |
| TDF | 163,940,526 | 163,940,547 | GCTGCCACATTCAGTCCTTTAG |
| TDP | 163,940,548 | 163,940,573 | TGGCCTGTCTCATTCTGCTGACACCT |
| TDR | 163,940,601 | 163,940,621 | AACACCAGACACTGCACATAC |
PCR Primers
| Primer | Sequence |
| SU | AACTGCACGTGACTGTTTGG |
| SD | AGACTAAGGGAACCATCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17495A-E7 | VGB6 | C57BL/6NTac | |
| 17495A-H11 | VGB6 | C57BL/6NTac | |
| 17495A-H2 | VGB6 | C57BL/6NTac | |
| 17495A-E9 | VGB6 | C57BL/6NTac | |
| 17495A-D1 | VGB6 | C57BL/6NTac | |
