| Gene | Selk |
| Chromosome | 14 |
| Deletion Start | 29,974,582 |
| Deletion End | 29,968,521 |
| Deletion Size | 6,062 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,968,769 | 29,968,790 | CGGGAATGACAGAGACGCTAAC |
| TUP | 29,968,791 | 29,968,812 | CCGGTCCGGATACAACGCGCTT |
| TUR | 29,968,838 | 29,968,861 | GGATGAAAGAGATGCTAGGCAAAG |
| TDF | 29,974,304 | 29,974,325 | TTTACCTCCTTGGACCTCCTTG |
| TDP | 29,974,329 | 29,974,354 | TCATGGCCAGCTCTCTTCACTGTTTC |
| TDR | 29,974,383 | 29,974,405 | TCTTTCTCTCTCTCTGGCATGTG |
PCR Primers
| Primer | Sequence |
| SU | CTAGCAAGCAACTTCCGGTC |
| SD | ACTCCAGGACTAAATACATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17498A-A8 | VGB6 | C57BL/6NTac | |
| 17498A-C5 | VGB6 | C57BL/6NTac | |
| 17498A-C10 | VGB6 | C57BL/6NTac | |
| 17498A-E12 | VGB6 | C57BL/6NTac | |
| 17498A-H10 | VGB6 | C57BL/6NTac | |
| 17498A-F2 | VGB6 | C57BL/6NTac | |
