| Gene | Serp1 |
| Chromosome | 3 |
| Deletion Start | 58,525,490 |
| Deletion End | 58,523,819 |
| Deletion Size | 1,672 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,525,443 | 58,525,464 | CAACGAGAAGCACAGCAAGAAC |
| TUP | 58,525,420 | 58,525,442 | ATAACTCAGCGCGGCAACGTCGC |
| TUR | 58,525,390 | 58,525,409 | GGGTGCCCACTTTCCCTTAC |
| TDF | 58,524,559 | 58,524,577 | ACTCTTGCCCCTGCGGATG |
| TDP | 58,524,528 | 58,524,553 | ATTACAGACTGTCCTCCTGCTCTGCC |
| TDR | 58,524,492 | 58,524,514 | TGTCTCAATAACAGCAGCTTGTC |
PCR Primers
| Primer | Sequence |
| SU | AGTCTGCGAGTCGGTGTTTC |
| SD | TGGAGTGTGCTTTCCAATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17500A-A6 | VGB6 | C57BL/6NTac | |
| 17500A-A10 | VGB6 | C57BL/6NTac | |
| 17500A-C11 | VGB6 | C57BL/6NTac | |
| 17500A-F2 | VGB6 | C57BL/6NTac | |
| 17500A-F8 | VGB6 | C57BL/6NTac | |
| 17500A-G2 | VGB6 | C57BL/6NTac | |
