| Gene | Srek1ip1 |
| Chromosome | 13 |
| Deletion Start | 104,837,594 |
| Deletion End | 104,817,357 |
| Deletion Size | 20,238 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,817,778 | 104,817,801 | ACCTGAGGCTTGTTCTAGCACTTC |
| TUP | 104,817,811 | 104,817,835 | ATTTCTATGTGGCTGCAGGCCCGCT |
| TUR | 104,817,855 | 104,817,879 | TGTCTTGAAACTGATAGGGAAACTG |
| TDF | 104,837,313 | 104,837,335 | GCAGATCCTACACAGTTGATGTC |
| TDP | 104,837,336 | 104,837,360 | TCCAGTCACAGCAGTGTTCTTGCCC |
| TDR | 104,837,365 | 104,837,385 | TCAGTGGCACTGGAGTAAGAC |
PCR Primers
| Primer | Sequence |
| SU | AAAGGGCCAATCCGTAAATC |
| SD | AAGAGCAAATCACTCCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17503A-A8 | VGB6 | C57BL/6NTac | |
| 17503A-C12 | VGB6 | C57BL/6NTac | |
