| Gene | Sgol2 |
| Chromosome | 1 |
| Deletion Start | 58,024,848 |
| Deletion End | 57,998,101 |
| Deletion Size | 26,748 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,998,723 | 57,998,745 | CATGTTGGCTACACTGGGAAATG |
| TUP | 57,998,749 | 57,998,773 | TGTCTGTCTGCTGATGTCACACCCA |
| TUR | 57,998,802 | 57,998,826 | GAGCAACGTCCTGACTTAAAGATAC |
| TDF | 58,024,624 | 58,024,645 | CAGTCGTGGGTGCTGTATAGTG |
| TDP | 58,024,647 | 58,024,670 | AGAGCAAAGCTCGGATGCTCAGGC |
| TDR | 58,024,682 | 58,024,701 | TCCTTCGACCCAGCTTTGTC |
PCR Primers
| Primer | Sequence |
| SU | CTGTCTTCTGCTGGTAGCCC |
| SD | TGTTAACAAGGCCTCCAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17505A-F9 | VGB6 | C57BL/6NTac | |
| 17505A-F8 | VGB6 | C57BL/6NTac | |
| 17505A-D1 | VGB6 | C57BL/6NTac | |
| 17505A-F5 | VGB6 | C57BL/6NTac | |
| 17505A-E1 | VGB6 | C57BL/6NTac | |
| 17505A-A8 | VGB6 | C57BL/6NTac | |
