| Gene | Slc25a38 |
| Chromosome | 9 |
| Deletion Start | 120,123,764 |
| Deletion End | 120,110,447 |
| Deletion Size | 13,318 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,111,347 | 120,111,370 | ACAAGAGTGCAGATGCCGAAGAAG |
| TUP | 120,111,379 | 120,111,405 | AGAACGTTGGTATCTCCTGGAGCTGGA |
| TUR | 120,111,419 | 120,111,439 | TCCCAGCACCCATACAGAAAG |
| TDF | 120,123,616 | 120,123,639 | AGCTTTCTCCCTTGTGTCCATCAC |
| TDP | 120,123,640 | 120,123,662 | AGAACCATGGGCTGCGTGGCTTC |
| TDR | 120,123,691 | 120,123,709 | GCAGCCATCAGCGTTCTTC |
PCR Primers
| Primer | Sequence |
| SU | GAGCAGAAGGCTCTGCAAC |
| SD | CTTGCTGAGGCATCTCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17507A-B1 | VGB6 | C57BL/6NTac | |
| 17507A-D8 | VGB6 | C57BL/6NTac | |
| 17507A-F2 | VGB6 | C57BL/6NTac | |
| 17507A-E3 | VGB6 | C57BL/6NTac | |
| 17507A-F4 | VGB6 | C57BL/6NTac | |
| 17507A-B5 | VGB6 | C57BL/6NTac | |
