| Gene | Sox17 |
| Chromosome | 1 |
| Deletion Start | 4,493,403 |
| Deletion End | 4,491,670 |
| Deletion Size | 1,734 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,493,164 | 4,493,183 | TTATGGTGTGGGCCAAAGAC |
| TUP | 4,493,141 | 4,493,161 | ACGCAAGCGGTTGGCACAGCA |
| TUR | 4,493,107 | 4,493,127 | CTTGCTTAGCTCTGCGTTGTG |
| TDF | 4,491,800 | 4,491,818 | ACACGACTGCGGAGTGAAC |
| TDP | 4,491,778 | 4,491,798 | TCTCAGACAGCCACGGAGCCA |
| TDR | 4,491,705 | 4,491,724 | GTCGGCAACCGTCAAATGTC |
PCR Primers
| Primer | Sequence |
| SU | AAGGCAGGTGTAGCCAGAAC |
| SD | CCGCTGTTTGTGTTTAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17509A-A5 | VGB6 | C57BL/6NTac | |
| 17509A-C4 | VGB6 | C57BL/6NTac | |
| 17509A-E6 | VGB6 | C57BL/6NTac | |
| 17509A-E2 | VGB6 | C57BL/6NTac | |
| 17509A-C5 | VGB6 | C57BL/6NTac | |
| 17509A-A7 | VGB6 | C57BL/6NTac | |
