| Gene | Spatc1 |
| Chromosome | 15 |
| Deletion Start | 76,292,516 |
| Deletion End | 76,268,175 |
| Deletion Size | 24,342 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,268,531 | 76,268,553 | CAGAGGCAGGATGAGAACATAGG |
| TUP | 76,268,555 | 76,268,581 | CCATTGGATGAAAGGCCAACCTAGGCC |
| TUR | 76,268,604 | 76,268,628 | GGTTGAAGGGAATGTAGGTAGGAAG |
| TDF | 76,292,280 | 76,292,298 | CCCGGCACTGACAGAACAG |
| TDP | 76,292,300 | 76,292,324 | TGGTCAATGAGTACGGCATCCTGCG |
| TDR | 76,292,371 | 76,292,389 | GCACACGCTGCAAGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | GTCAAGACATTGCAGGCTG |
| SD | CTTTCTGCATCATCCCTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17510A-B1 | VGB6 | C57BL/6NTac | |
| 17510A-B12 | VGB6 | C57BL/6NTac | |
| 17510A-G12 | VGB6 | C57BL/6NTac | |
| 17510A-D4 | VGB6 | C57BL/6NTac | |
| 17510A-E5 | VGB6 | C57BL/6NTac | |
| 17510A-E10 | VGB6 | C57BL/6NTac | |
