| Gene | Stard8 |
| Chromosome | X |
| Deletion Start | 99,073,226 |
| Deletion End | 99,064,404 |
| Deletion Size | 8,823 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,064,732 | 99,064,752 | ACCTCCACTGCTTGGTTTGTC |
| TUP | 99,064,761 | 99,064,790 | AATTCCTTGAACAGGAGAAGGGACGGGACA |
| TUR | 99,064,799 | 99,064,819 | CCATCGAGCCAATGACCTTTC |
| TDF | 99,072,321 | 99,072,342 | GGAGCCCAGTAGCCATAAGAAG |
| TDP | 99,072,345 | 99,072,367 | AGTGCCAGGCCCTGCAGTCTGAT |
| TDR | 99,072,403 | 99,072,426 | CCAAATGGGTAGGGATAGGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | TCTCAGCATGACACCTTTGG |
| SD | CAAAGGCAGGATCAGACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17514A-B11 | VGB6 | C57BL/6NTac | |
| 17514A-E6 | VGB6 | C57BL/6NTac | |
| 17514A-E11 | VGB6 | C57BL/6NTac | |
| 17514A-E12 | VGB6 | C57BL/6NTac | |
| 17514A-H8 | VGB6 | C57BL/6NTac | |
| 17514A-F11 | VGB6 | C57BL/6NTac | |
