| Gene | Stim2 |
| Chromosome | 5 |
| Deletion Start | 53,999,031 |
| Deletion End | 53,998,689 |
| Deletion Size | 343 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,998,722 | 53,998,740 | GGTGAAGGGAGCGGGTCAG |
| TUP | 53,998,742 | 53,998,761 | ACCCGGCGGGAGTCCGCTTC |
| TUR | 53,998,782 | 53,998,799 | CTCGCTCACCGCCAGCTG |
| TDF | 53,998,880 | 53,998,901 | AGCAATGCTGCTCTTCGGGTTG |
| TDP | | | TTGGTGGCCGGCGTGGC |
| TDR | 53,998,923 | 53,998,940 | GGGCACCAGATCGCATCC |
PCR Primers
| Primer | Sequence |
| SU | CGCTTTATCCAGGTCTCCAG |
| SD | TGGCGGCTTTGGGAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 61 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17516A-H9 | VGB6 | C57BL/6NTac | |
| 17516A-E2 | VGB6 | C57BL/6NTac | |
| 17516A-D7 | VGB6 | C57BL/6NTac | |
| 17516A-H1 | VGB6 | C57BL/6NTac | |
