| Gene | Timm10 |
| Chromosome | 2 |
| Deletion Start | 84,830,013 |
| Deletion End | 84,827,646 |
| Deletion Size | 2,368 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,827,823 | 84,827,842 | CAAGTCCCGCCCTCCTAATC |
| TUP | 84,827,850 | 84,827,870 | ACCCTCACCGCGTGCTTCGCT |
| TUR | 84,827,895 | 84,827,917 | CCCTCCAAATAGCCAACCAAGTC |
| TDF | 84,829,715 | 84,829,736 | AGGGTTGGGCTTGTTGAAGGTG |
| TDP | 84,829,739 | 84,829,762 | TGGGATGCTCCAGGAAGTCACTGA |
| TDR | 84,829,789 | 84,829,806 | GGCAGGCACTGGTCATTC |
PCR Primers
| Primer | Sequence |
| SU | CCCAACTGTCAATCCTCTGG |
| SD | ACCCACTGCATTAAATTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17522A-B12 | VGB6 | C57BL/6NTac | |
| 17522A-C1 | VGB6 | C57BL/6NTac | |
| 17522A-D7 | VGB6 | C57BL/6NTac | |
| 17522A-D8 | VGB6 | C57BL/6NTac | |
| 17522A-F7 | VGB6 | C57BL/6NTac | |
