| Gene | Tle6 |
| Chromosome | 10 |
| Deletion Start | 81,600,076 |
| Deletion End | 81,591,008 |
| Deletion Size | 9,069 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,599,197 | 81,599,218 | GGCTGGAAGCTAGACATGACTC |
| TUP | 81,599,171 | 81,599,194 | CACCTGGGACTTCAGGGAGATGCA |
| TUR | 81,599,130 | 81,599,149 | GGTGGAAGGCTGGAAAGGTC |
| TDF | 81,591,672 | 81,591,694 | TCTCAGAGGACCCTGCTATTTAC |
| TDP | 81,591,650 | 81,591,671 | TCAGTGCCCAGTCAGGGCTTGG |
| TDR | 81,591,575 | 81,591,596 | CCCAAGTAGCCAGAGATGACTC |
PCR Primers
| Primer | Sequence |
| SU | GCTAGGGAGTTGGAGGTGC |
| SD | TGGGCTTTACCAACTGAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 120 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17523A-B3 | VGB6 | C57BL/6NTac | |
| 17523A-H4 | VGB6 | C57BL/6NTac | |
| 17523A-G10 | VGB6 | C57BL/6NTac | |
| 17523A-F7 | VGB6 | C57BL/6NTac | |
| 17523A-D5 | VGB6 | C57BL/6NTac | |
| 17523A-H10 | VGB6 | C57BL/6NTac | |
