| Gene | Tnk2 |
| Chromosome | 16 |
| Deletion Start | 32,682,734 |
| Deletion End | 32,663,760 |
| Deletion Size | 18,975 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,664,668 | 32,664,688 | CCAGTTTCGTTCCCACATCAG |
| TUP | 32,664,691 | 32,664,716 | AGCTTGTAACTGCCAGTAGCTCCACA |
| TUR | 32,664,729 | 32,664,747 | TGCCCATAGGGACCAGAAG |
| TDF | 32,682,370 | 32,682,391 | GCAAACATAGTGTGGCCCTCAG |
| TDP | 32,682,392 | 32,682,420 | CAAGCAGCGACACCCTTTACTAAGCTGTA |
| TDR | 32,682,426 | 32,682,444 | GGGACAACCACCAGCAGAG |
PCR Primers
| Primer | Sequence |
| SU | TGCCTCACAGTCCTTCATTG |
| SD | TGTCCAACACTCCACACTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17524A-H7 | VGB6 | C57BL/6NTac | |
| 17524A-A11 | VGB6 | C57BL/6NTac | |
| 17524A-G11 | VGB6 | C57BL/6NTac | |
| 17524A-F4 | VGB6 | C57BL/6NTac | |
| 17524A-A6 | VGB6 | C57BL/6NTac | |
