| Gene | Crh |
| Chromosome | 3 |
| Deletion Start | 19,694,473 |
| Deletion End | 19,693,831 |
| Deletion Size | 643 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,694,333 | 19,694,350 | TTCTTGCAGCCGGAGCAG |
| TUP | 19,694,310 | 19,694,331 | CCCAGCAACCTCAGCCGGTTCT |
| TUR | 19,694,251 | 19,694,273 | CAGCGGGACTTCTGTTGAGATTC |
| TDF | 19,693,982 | 19,694,002 | CTGCGGGAAGTCTTGGAAATG |
| TDP | 19,693,959 | 19,693,980 | CCCGGGCAGAGCAGTTAGCTCA |
| TDR | 19,693,930 | 19,693,951 | CCATCAGTTTCCTGTTGCTGTG |
PCR Primers
| Primer | Sequence |
| SU | GGGAAGTCGGTTTAGGGAAG |
| SD | CACAACCAAATTGACCAAGAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17526A-A11 | VGB6 | C57BL/6NTac | |
| 17526B-F7 | VGB6 | C57BL/6NTac | |
