| Gene | Acta2 |
| Chromosome | 19 |
| Deletion Start | 34,252,550 |
| Deletion End | 34,241,660 |
| Deletion Size | 10,891 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,252,494 | 34,252,515 | CAATGGCTCTGGGCTCTGTAAG |
| TUP | 34,252,471 | 34,252,492 | CCGGCTTCGCTGGTGATGATGC |
| TUR | 34,252,392 | 34,252,411 | TCCTCTCCGCGTTCAATGTG |
| TDF | 34,242,263 | 34,242,284 | AGGCCAGCATGACAGGAGATAC |
| TDP | 34,242,238 | 34,242,262 | ACCGGAATGGGAACTCTGAGCCCTC |
| TDR | 34,242,218 | 34,242,236 | CATAAGCACCGCCCAACAC |
PCR Primers
| Primer | Sequence |
| SU | TTTGTGCTGAGGACTTGTGG |
| SD | GGACCTTCCTCTGTTGAAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 124 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17527A-A2 | VGB6 | C57BL/6NTac | |
| 17527A-H1 | VGB6 | C57BL/6NTac | |
| 17527A-H8 | VGB6 | C57BL/6NTac | |
| 17527A-C11 | VGB6 | C57BL/6NTac | |
| 17527A-D1 | VGB6 | C57BL/6NTac | |
