| Gene | Ntf5 |
| Chromosome | 7 |
| Deletion Start | 45,416,128 |
| Deletion End | 45,415,449 |
| Deletion Size | 680 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,415,538 | 45,415,556 | CCCATTTCTGGCCCCTGAG |
| TUP | 45,415,557 | 45,415,577 | TGGGACCTCTTGTCTCCCCGA |
| TUR | 45,415,617 | 45,415,634 | GGCCTCCAGCAGGAAGAG |
| TDF | 45,415,950 | 45,415,971 | TGCAAGGCTAAGCAGTCCTATG |
| TDP | 45,415,974 | 45,415,995 | CGGGCGTTGACTGCAGACTCCC |
| TDR | 45,416,015 | 45,416,035 | GCTGTGTCGATCCGAATCCAG |
PCR Primers
| Primer | Sequence |
| SU | ACTTCTTGGCTGTGTGACCC |
| SD | ATGATGTTCAGGATGGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17529A-B8 | VGB6 | C57BL/6NTac | |
| 17529A-E1 | VGB6 | C57BL/6NTac | |
| 17529A-F11 | VGB6 | C57BL/6NTac | |
| 17529A-G4 | VGB6 | C57BL/6NTac | |
| 17529A-G10 | VGB6 | C57BL/6NTac | |
| 17529A-A7 | VGB6 | C57BL/6NTac | |
