| Gene | Caprin2 |
| Chromosome | 6 |
| Deletion Start | 148,895,110 |
| Deletion End | 148,868,993 |
| Deletion Size | 26,118 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,894,914 | 148,894,936 | GTAACCTTTCTACCCCTCCCATC |
| TUP | 148,894,886 | 148,894,913 | CCTGAGTGTGCATTACCATAAGCCAGGT |
| TUR | 148,894,806 | 148,894,827 | TCCCAGGCAACTAAGTCTCAAG |
| TDF | 148,869,388 | 148,869,411 | CCAACATTGCAGGAAGAGCAGAAG |
| TDP | 148,869,350 | 148,869,379 | TCCTATAACACCTGTCAGTCAGTGGAAGCC |
| TDR | 148,869,320 | 148,869,341 | GGACTGCCCACTTTGGACTTAG |
PCR Primers
| Primer | Sequence |
| SU | TAGCTTGAAGTTGTGGCTGC |
| SD | CTTGATCAGAAAGTTCCTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 131 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17530A-A12 | VGB6 | C57BL/6NTac | |
| 17530A-C10 | VGB6 | C57BL/6NTac | |
