| Gene | Crhr1 |
| Chromosome | 11 |
| Deletion Start | 104,174,566 |
| Deletion End | 104,133,075 |
| Deletion Size | 41,492 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,133,970 | 104,133,990 | GCTGACATGGACCTCCTGAAC |
| TUP | 104,133,991 | 104,134,014 | CACAAGCCGAAGCACCGGGTTAAC |
| TUR | 104,134,048 | 104,134,067 | TGCTCCTCCCAACCACTTAG |
| TDF | 104,173,975 | 104,173,998 | GGACTGTCTCCATCATATCTCTTC |
| TDP | 104,173,999 | 104,174,018 | TGCCGTGGGCTGGCTTTCCT |
| TDR | 104,174,052 | 104,174,071 | CAGGGACGCTCTCAAGGTTC |
PCR Primers
| Primer | Sequence |
| SU | GCAGTGGACCGAGGAGAC |
| SD | ACCATGTTGGTCCTCGGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17531A-B3 | VGB6 | C57BL/6NTac | |
| 17531A-E12 | VGB6 | C57BL/6NTac | |
| 17531A-D8 | VGB6 | C57BL/6NTac | |
| 17531A-B8 | VGB6 | C57BL/6NTac | |
