| Gene | Gmeb1 |
| Chromosome | 4 |
| Deletion Start | 132,251,799 |
| Deletion End | 132,225,751 |
| Deletion Size | 26,049 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 132,251,670 | 132,251,690 | AGCCTGTGCAACAAGGGTGAG |
| TUP | 132,251,641 | 132,251,669 | TGGCTAGCAATTTGGCTATTCCAGACAGT |
| TUR | 132,251,605 | 132,251,628 | GCTCCTACTTCCCATCCTTTCAAC |
| TDF | 132,226,149 | 132,226,166 | TCTGGCCCTCAGCTCTTC |
| TDP | 132,226,126 | 132,226,148 | CGCTATGCCACGGTGGTTTCCTC |
| TDR | 132,226,102 | 132,226,122 | GTGTCTGGTGAGTTGCTCTTG |
PCR Primers
| Primer | Sequence |
| SU | AGTGCTGTTCATTTCTGGGC |
| SD | TGTATCCACAGCAGAGCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17532A-C9 | VGB6 | C57BL/6NTac | |
| 17532A-C11 | VGB6 | C57BL/6NTac | |
| 17532A-E10 | VGB6 | C57BL/6NTac | |
