| Gene | Egln2 |
| Chromosome | 7 |
| Deletion Start | 27,165,495 |
| Deletion End | 27,159,058 |
| Deletion Size | 6,438 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,164,686 | 27,164,706 | ATTGGTGCCCTCATGGCTCAC |
| TUP | 27,164,662 | 27,164,684 | TGGACGCAGTAATCCGCCACTGT |
| TUR | 27,164,626 | 27,164,644 | GCGCCCATTGATGACGTAG |
| TDF | 27,159,435 | 27,159,453 | TGGGTGCTGAGAGTGGTTC |
| TDP | 27,159,411 | 27,159,433 | CAGGGCCTTTGCCACACTAGGCC |
| TDR | 27,159,361 | 27,159,383 | GCAAAGGGACTGGTGAGAAAGAC |
PCR Primers
| Primer | Sequence |
| SU | CCATGTTGTCTCCCTGTGTG |
| SD | CAGAACCTCCATACCCATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17533A-A8 | VGB6 | C57BL/6NTac | |
| 17533A-E6 | VGB6 | C57BL/6NTac | |
| 17533A-C10 | VGB6 | C57BL/6NTac | |
| 17533A-D4 | VGB6 | C57BL/6NTac | |
| 17533A-C11 | VGB6 | C57BL/6NTac | |
| 17533A-B4 | VGB6 | C57BL/6NTac | |
