| Gene | Plin3 |
| Chromosome | 17 |
| Deletion Start | 56,287,856 |
| Deletion End | 56,279,715 |
| Deletion Size | 8,142 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,287,087 | 56,287,108 | AGCCCAGGACATCAAAGTCATC |
| TUP | 56,287,061 | 56,287,084 | AGAACCCTTGAGGGTCACAGCCTG |
| TUR | 56,286,995 | 56,287,015 | AACAGGCCCTTGGTTCCATTC |
| TDF | 56,279,888 | 56,279,906 | TCACTCCTTCCAGGACCTC |
| TDP | 56,279,866 | 56,279,886 | CAGCCGGCGTTCTTGCCCAAA |
| TDR | 56,279,810 | 56,279,830 | CACGTACTCCACAGTGTTGTC |
PCR Primers
| Primer | Sequence |
| SU | TTGGCACTTCTCCAGCACC |
| SD | TTTTCTAGAATGTTCTGGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17534A-B5 | VGB6 | C57BL/6NTac | |
| 17534A-B9 | VGB6 | C57BL/6NTac | |
| 17534A-B10 | VGB6 | C57BL/6NTac | |
| 17534A-E6 | VGB6 | C57BL/6NTac | |
| 17534A-G12 | VGB6 | C57BL/6NTac | |
| 17534A-C1 | VGB6 | C57BL/6NTac | |
