| Gene | Rheb |
| Chromosome | 5 |
| Deletion Start | 24,842,233 |
| Deletion End | 24,803,627 |
| Deletion Size | 38,607 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,841,718 | 24,841,739 | CGCCTCCAAGAAACCTAGACTG |
| TUP | 24,841,690 | 24,841,714 | CCCTCACAGGCCAGCTAGGTAGCAT |
| TUR | 24,841,661 | 24,841,681 | TTTCTCCTCTTCCCTGTGCTG |
| TDF | 24,804,234 | 24,804,251 | CTTGGCCTGACGGGTTTG |
| TDP | 24,804,210 | 24,804,232 | AATTGGCGGCATGGTACACAGGA |
| TDR | 24,804,185 | 24,804,206 | GTCCTCCATCACCCACAGTTAG |
PCR Primers
| Primer | Sequence |
| SU | GTCATGACGCAGCGAGTTTC |
| SD | CACACCAAGTAGCTCAAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17537A-A5 | VGB6 | C57BL/6NTac | |
| 17537A-D2 | VGB6 | C57BL/6NTac | |
| 17537A-D5 | VGB6 | C57BL/6NTac | |
| 17537A-G12 | VGB6 | C57BL/6NTac | |
| 17537A-F9 | VGB6 | C57BL/6NTac | |
| 17537A-D9 | VGB6 | C57BL/6NTac | |
