| Gene | Pten |
| Chromosome | 19 |
| Deletion Start | 32,776,094 |
| Deletion End | 32,758,448 |
| Deletion Size | 17,647 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,758,493 | 32,758,516 | CAAGAGGATGGATTCGACTTAGAC |
| TUP | 32,758,518 | 32,758,543 | TGACCTGTATCCATTTCTGCGGCTGT |
| TUR | 32,758,558 | 32,758,579 | TCCCACGTTATCAGAGTGACAG |
| TDF | 32,775,783 | 32,775,806 | AGCTGTCAGGGATGAGGTTATCAC |
| TDP | 32,775,810 | 32,775,831 | CCGCCGAGTCACTGTGGGCAGT |
| TDR | 32,775,846 | 32,775,868 | TCAGCCACTCACTATCCTTAGAC |
PCR Primers
| Primer | Sequence |
| SU | TGCGAGGATTATCCGTCTTC |
| SD | GAGAATAACCTGGTAAGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17539A-H11 | VGB6 | C57BL/6NTac | |
| 17539A-F11 | VGB6 | C57BL/6NTac | |
