| Gene | Uevld |
| Chromosome | 7 |
| Deletion Start | 46,958,429 |
| Deletion End | 46,926,310 |
| Deletion Size | 32,120 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,958,085 | 46,958,104 | ATTCTCCGCCGTGCTTCCTC |
| TUP | 46,958,057 | 46,958,083 | TGGAGCGTTTGTGACTGTACACTGTAC |
| TUR | 46,958,017 | 46,958,038 | TGTGACCACAGTGCTAGATGAG |
| TDF | 46,926,730 | 46,926,750 | GGCAGTGGTTGCTTGTATGAG |
| TDP | 46,926,704 | 46,926,729 | TGCAGGTGCCCATGAACTCTAAAGAC |
| TDR | 46,926,669 | 46,926,692 | CAGCCGCCTATAACTCTAGCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TGCATCTGTTCGTTCTTTGG |
| SD | ATCCTGCCTTCAGCTCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17544A-D4 | VGB6 | C57BL/6NTac | |
| 17544A-H1 | VGB6 | C57BL/6NTac | |
