| Gene | Vdac1 |
| Chromosome | 11 |
| Deletion Start | 52,388,615 |
| Deletion End | 52,374,305 |
| Deletion Size | 14,311 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,374,358 | 52,374,377 | CAAGGGCTACGGTGAGTGTC |
| TUP | 52,374,383 | 52,374,408 | AGTCGGGAGCAATGGGAAGCCTTTCA |
| TUR | 52,374,429 | 52,374,450 | GACCACCTCAAGTGTCACAAAG |
| TDF | 52,388,225 | 52,388,245 | GGATAGCAGCAGGTCAGGTTG |
| TDP | 52,388,249 | 52,388,273 | TACAGAGCACACCTTGAAGGGTCGC |
| TDR | 52,388,284 | 52,388,302 | CCAGGTGCAGTCAGCAGTG |
PCR Primers
| Primer | Sequence |
| SU | CTGGGAATATGTCTTGGATG |
| SD | AGTGATGGGCTAGGATGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17546A-B5 | VGB6 | C57BL/6NTac | |
| 17546A-B7 | VGB6 | C57BL/6NTac | |
| 17546A-C10 | VGB6 | C57BL/6NTac | |
| 17546A-G3 | VGB6 | C57BL/6NTac | |
| 17546A-G8 | VGB6 | C57BL/6NTac | |
| 17546A-D1 | VGB6 | C57BL/6NTac | |
