| Gene | Vim |
| Chromosome | 2 |
| Deletion Start | 13,582,615 |
| Deletion End | 13,574,435 |
| Deletion Size | 8,181 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,574,761 | 13,574,782 | GAATGACCGCTTTGCCAACTAC |
| TUP | 13,574,784 | 13,574,805 | TCGACAAGGTGCGCTTCCTCGA |
| TUR | 13,574,843 | 13,574,861 | ACTTGCCCTGGCCCTTGAG |
| TDF | 13,582,295 | 13,582,316 | GTCACACCTTTCCACTGGATAC |
| TDP | 13,582,318 | 13,582,343 | TGTATTGTCTGCAGGAAGAGGCCACC |
| TDR | 13,582,359 | 13,582,377 | TGCAGAACGAGGCCCTAAG |
PCR Primers
| Primer | Sequence |
| SU | GCACCCAAGGGTGAGTAGAG |
| SD | TCGCAAGCATAAATAAATTGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17547A-H2 | VGB6 | C57BL/6NTac | |
| 17547A-G2 | VGB6 | C57BL/6NTac | |
| 17547A-F3 | VGB6 | C57BL/6NTac | |
