| Gene | Wars |
| Chromosome | 12 |
| Deletion Start | 108,888,483 |
| Deletion End | 108,860,242 |
| Deletion Size | 28,242 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,888,348 | 108,888,366 | TTGTCGGGCTCTCCGAAAC |
| TUP | 108,888,326 | 108,888,347 | ATGCACCTCAGCCACCACGCCT |
| TUR | 108,888,268 | 108,888,291 | GCCTCAGAAGCACATAAGAAGGAC |
| TDF | 108,861,215 | 108,861,235 | AACCCACCCTGTCAAATCAAG |
| TDP | 108,861,187 | 108,861,213 | CACACTCCCAATAGGCTTCTCACGTGG |
| TDR | 108,861,126 | 108,861,149 | CATCAGAGAAGAAACAGCATCCAG |
PCR Primers
| Primer | Sequence |
| SU | TCTGGGCACTAGATGGGAAC |
| SD | CACAATGATCCCTAGGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17548A-A10 | VGB6 | C57BL/6NTac | |
| 17548A-H3 | VGB6 | C57BL/6NTac | |
