| Gene | Wbscr16 |
| Chromosome | 5 |
| Deletion Start | 134,176,711 |
| Deletion End | 134,148,851 |
| Deletion Size | 27,861 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,176,286 | 134,176,303 | TGCAGAGGGAGGCATGAG |
| TUP | 134,176,258 | 134,176,282 | TGGCTGCCGAGAAGTCTGTTTCTCC |
| TUR | 134,176,205 | 134,176,229 | CCCAAGATCCACAGACGTGATAAAC |
| TDF | 134,149,260 | 134,149,279 | GGGTGAGGCTCAATGGGTAG |
| TDP | 134,149,231 | 134,149,257 | TGCTTGCCTAACACATACAAAGCCTGA |
| TDR | 134,149,207 | 134,149,228 | TTACGTGGTATTGGAGACAGAC |
PCR Primers
| Primer | Sequence |
| SU | AAGGATGTGTGAGGACCTGG |
| SD | AGTCAGCTGGCTTCTTGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17549A-A11 | VGB6 | C57BL/6NTac | |
| 17549A-C7 | VGB6 | C57BL/6NTac | |
| 17549A-H2 | VGB6 | C57BL/6NTac | |
| 17549A-D7 | VGB6 | C57BL/6NTac | |
| 17549A-E7 | VGB6 | C57BL/6NTac | |
| 17549A-E1 | VGB6 | C57BL/6NTac | |
