| Gene | Zfp553 |
| Chromosome | 7 |
| Deletion Start | 127,237,135 |
| Deletion End | 127,235,278 |
| Deletion Size | 1,858 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,235,988 | 127,236,006 | ACCCAAGGCCCAGGACAAG |
| TUP | 127,236,008 | 127,236,031 | CGTATATCTGCACCGACTGCGGCA |
| TUR | 127,236,058 | 127,236,076 | GACTCCGTTGGTGGCTCAG |
| TDF | 127,236,265 | 127,236,283 | GCCTGCCCAGAGTGTAACC |
| TDP | 127,236,289 | 127,236,315 | TTTAGCCTCAGCTCCACTCTTCTTCGC |
| TDR | 127,236,345 | 127,236,364 | GGTAGGCTGCGAGACTGAAG |
PCR Primers
| Primer | Sequence |
| SU | TAAGGATTGCGGCACAGTAG |
| SD | TTCCAACAATCTGCTCTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17558A-A7 | VGB6 | C57BL/6NTac | |
| 17558A-G5 | VGB6 | C57BL/6NTac | |
| 17558A-C4 | VGB6 | C57BL/6NTac | |
| 17558A-G1 | VGB6 | C57BL/6NTac | |
| 17558A-F8 | VGB6 | C57BL/6NTac | |
| 17558A-F4 | VGB6 | C57BL/6NTac | |
