| Gene | Zfp623 |
| Chromosome | 15 |
| Deletion Start | 75,948,701 |
| Deletion End | 75,947,200 |
| Deletion Size | 1,502 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,947,654 | 75,947,678 | GGAAAGACTTCGTTCACTACGCAAG |
| TUP | 75,947,679 | 75,947,702 | CCTGAGGGAGCATCAGCATGTCCA |
| TUR | 75,947,752 | 75,947,772 | TCGAAGCAGGTCTGAACTGTG |
| TDF | 75,948,092 | 75,948,114 | GCTCGAAACTCATTCAGCACCAG |
| TDP | 75,948,119 | 75,948,141 | TTCACACGGGAGAGCGGCCTTAC |
| TDR | 75,948,142 | 75,948,160 | TCCCACACTCGTTGCACAC |
PCR Primers
| Primer | Sequence |
| SU | CTGTCACCCACACAGGACAC |
| SD | ATGAGTCCCAGACAACAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 119 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17559A-A8 | VGB6 | C57BL/6NTac | |
| 17559A-F11 | VGB6 | C57BL/6NTac | |
| 17559A-F12 | VGB6 | C57BL/6NTac | |
| 17559A-E4 | VGB6 | C57BL/6NTac | |
