| Gene | Zfp777 |
| Chromosome | 6 |
| Deletion Start | 48,048,099 |
| Deletion End | 48,024,628 |
| Deletion Size | 23,472 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,047,287 | 48,047,308 | ATTAAACGCCCTCCCACCCAAC |
| TUP | 48,047,262 | 48,047,282 | TCTCTGAGGCACCCTGGCTGT |
| TUR | 48,047,218 | 48,047,239 | ACAGGTTCAAAGGCACGGACTC |
| TDF | 48,025,386 | 48,025,404 | CCTACGAGTGTGCCGAGTG |
| TDP | 48,025,361 | 48,025,385 | TGAGATCAGCTTCCGCCACAAGCAG |
| TDR | 48,025,342 | 48,025,360 | GCTGGTGCAGCGTGAGTTG |
PCR Primers
| Primer | Sequence |
| SU | CAGCTCCCCTGCTGAAGAG |
| SD | ACTAAATGGCCCTGCATCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17562A-A9 | VGB6 | C57BL/6NTac | |
| 17562A-B4 | VGB6 | C57BL/6NTac | |
| 17562A-C2 | VGB6 | C57BL/6NTac | |
| 17562A-G9 | VGB6 | C57BL/6NTac | |
