| Gene | Zfp819 |
| Chromosome | 7 |
| Deletion Start | 43,617,965 |
| Deletion End | 43,609,910 |
| Deletion Size | 8,056 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,610,044 | 43,610,066 | TGTGAGTTCCCTTCCTGGGATAG |
| TUP | 43,610,068 | 43,610,091 | CTTGATCCTCCAGCTAAGGGCGGA |
| TUR | 43,610,097 | 43,610,115 | GGCTGGGATTTCCGCTGTC |
| TDF | 43,617,414 | 43,617,435 | TGAGTGCAGAGATTGTGGGAAG |
| TDP | 43,617,448 | 43,617,474 | AAATCTCACCTGCCTGTGCACTATCGC |
| TDR | 43,617,489 | 43,617,509 | TCGCAGCATCTGTAAGGTTTC |
PCR Primers
| Primer | Sequence |
| SU | TTGTTTGTTTCCTGGATCTTTG |
| SD | AGCAGCAATGGATGAATGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17563A-B9 | VGB6 | C57BL/6NTac | |
| 17563A-C5 | VGB6 | C57BL/6NTac | |
| 17563A-F2 | VGB6 | C57BL/6NTac | |
| 17563A-H2 | VGB6 | C57BL/6NTac | |
| 17563A-F9 | VGB6 | C57BL/6NTac | |
| 17563A-E2 | VGB6 | C57BL/6NTac | |
