17564   Cwc25     Status: ES cell colonies screened / QC positives

GeneCwc25
Chromosome11
Deletion Start97,766,439
Deletion End97,747,322
Deletion Size19,118
Genome Build38
MGI IDMGI:1914730
NCBI Gene ID67480
BAC ID165E22
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,765,98997,766,008TGAAAGGGTGGGCTTCAGTC
TUP97,765,96597,765,986TTCCCATCCTGAGCTGTGCCGT
TUR97,765,88097,765,900GCTGCCTCCTCACTACACATC
TDF97,748,26097,748,281GAGGCATCAGAAGGAAGGAAAC
TDP97,748,23797,748,259TCACCTAAGCATGGCGTTGCTCC
TDR97,748,19497,748,212CATGCAGGCAGGTCACAGG

PCR Primers

PrimerSequence
SUCGGCCTAAGTTGGCAGATAC
SDGATCCATTCATTCAGGTGGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR129 bp++-
TDFTDR88 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17564A-B9VGB6C57BL/6NTac
17564A-B11VGB6C57BL/6NTac
17564A-C6VGB6C57BL/6NTac
17564A-G11VGB6C57BL/6NTac
17564A-F2VGB6C57BL/6NTac
17564A-D4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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