| Gene | Zcchc4 |
| Chromosome | 5 |
| Deletion Start | 52,808,355 |
| Deletion End | 52,783,094 |
| Deletion Size | 25,262 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,783,472 | 52,783,494 | GGGAGAAAGCCGAACGTAGAATG |
| TUP | 52,783,503 | 52,783,532 | TGAAGGCTTCTTGCTGACAAGTTCTTAGCG |
| TUR | 52,783,585 | 52,783,605 | TAGGCTTCCAGGGTTTTGTTG |
| TDF | 52,807,621 | 52,807,643 | GAGGGCTGACTATTGGCTTGTTC |
| TDP | 52,807,646 | 52,807,668 | CATGGCTTCCTCGGCCTGCTCTT |
| TDR | 52,807,671 | 52,807,690 | CTGGCGGCCATGAGTTGTAG |
PCR Primers
| Primer | Sequence |
| SU | AACGCAATAGAGCTGCTGTG |
| SD | GAGTGTGTGTACTCAAGAATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 134 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17568A-H4 | VGB6 | C57BL/6NTac | |
| 17568C-D2 | VGB6 | C57BL/6NTac | |
