17571   Arl6ip5     Status: ES cell colonies screened / QC positives

GeneArl6ip5
Chromosome6
Deletion Start97,232,666
Deletion End97,210,824
Deletion Size21,843
Genome Build38
MGI IDMGI:1929501
NCBI Gene ID65106
BAC ID424J5
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,211,38397,211,403CCTAGCAAAGCCTGGAGTGAC
TUP97,211,40897,211,430TGTGGACCCAGGGCTGTGTTTAA
TUR97,211,43697,211,456AGACAGACATTAGCCGGAGAG
TDF97,232,13797,232,159GGGATTATTGGGCTGGTGAGATG
TDP97,232,16997,232,195TTAAGAGCACAGAGGACCCAAGGTTGG
TDR97,232,21097,232,228TGGTGCTGAGCCACCTAAC

PCR Primers

PrimerSequence
SUCACTGCTCTGAGTCACTAAC
SDACAGGTGCCCAAGTGATAGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR74 bp++-
TDFTDR92 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17571A-B6VGB6C57BL/6NTac
17571A-B10VGB6C57BL/6NTac
17571A-D9VGB6C57BL/6NTac
17571A-G12VGB6C57BL/6NTac
17571A-D12VGB6C57BL/6NTac
17571A-H12VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image