| Gene | Arvcf |
| Chromosome | 16 |
| Deletion Start | 18,405,262 |
| Deletion End | 18,389,181 |
| Deletion Size | 16,082 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,389,710 | 18,389,734 | GGGCAGTTCCTTTAAGAGTGTAGTC |
| TUP | 18,389,739 | 18,389,761 | CAGGCATGATCCCACCATCTGCC |
| TUR | 18,389,769 | 18,389,791 | GGGAAACTGATGCTAGGTCACAG |
| TDF | 18,404,472 | 18,404,490 | CAGTTGACCGGAGGGAGAG |
| TDP | 18,404,492 | 18,404,515 | AGGACACTGGGCAGTGACTCCACA |
| TDR | 18,404,550 | 18,404,569 | GGACACTTGCTTGCTGTTAC |
PCR Primers
| Primer | Sequence |
| SU | CTATGGCTCAGCTGTGCTTG |
| SD | GCAAAGGAAGGCAAAGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17574A-C6 | VGB6 | C57BL/6NTac | |
| 17574A-E6 | VGB6 | C57BL/6NTac | |
| 17574A-G1 | VGB6 | C57BL/6NTac | |
| 17574A-G3 | VGB6 | C57BL/6NTac | |
| 17574A-E4 | VGB6 | C57BL/6NTac | |
