| Gene | BC031781 |
| Chromosome | 1 |
| Deletion Start | 180,866,294 |
| Deletion End | 180,851,176 |
| Deletion Size | 15,119 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 180,851,555 | 180,851,575 | TGCTTTCTTACCGACCCTCAG |
| TUP | 180,851,578 | 180,851,601 | CACCCATCCAGGCCAAAGAACGGT |
| TUR | 180,851,616 | 180,851,635 | TGGCTCTGTCAGGATGACTC |
| TDF | 180,865,457 | 180,865,475 | GTGGAAGCACGTTGGTCTC |
| TDP | 180,865,479 | 180,865,505 | ACGTAGCTAGTACTGAACGCTCGAAGC |
| TDR | 180,865,516 | 180,865,536 | TGGGTAGCCACTCTTACTCTG |
PCR Primers
| Primer | Sequence |
| SU | AATAGCTGCACAAACCGGAG |
| SD | ACCTCAAGTTCAGGTACCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17575A-C10 | VGB6 | C57BL/6NTac | |
| 17575A-D9 | VGB6 | C57BL/6NTac | |
| 17575A-D10 | VGB6 | C57BL/6NTac | |
| 17575A-E5 | VGB6 | C57BL/6NTac | |
| 17575A-E7 | VGB6 | C57BL/6NTac | |
| 17575A-H11 | VGB6 | C57BL/6NTac | |
