| Gene | Bccip |
| Chromosome | 7 |
| Deletion Start | 133,720,920 |
| Deletion End | 133,709,361 |
| Deletion Size | 11,560 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,709,521 | 133,709,540 | TGGACGAGGTGAGAAAGCAC |
| TUP | 133,709,542 | 133,709,564 | CGTGACCATTTCAGGCAGCCGAA |
| TUR | 133,709,565 | 133,709,585 | CTCCCATTACGTTCCCACAAC |
| TDF | 133,720,531 | 133,720,554 | CTGTGAGTTGAAATGTGTTTGGTG |
| TDP | 133,720,558 | 133,720,583 | CATCATGTCTCCCTGAAGTGTGCGTC |
| TDR | 133,720,605 | 133,720,623 | AACAGCGGAGGGAAGGAAC |
PCR Primers
| Primer | Sequence |
| SU | GATGGGCCAAACCACTACAC |
| SD | GCCTCCTAGTGAAAGCAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17576A-A9 | VGB6 | C57BL/6NTac | |
| 17576A-B8 | VGB6 | C57BL/6NTac | |
| 17576A-B10 | VGB6 | C57BL/6NTac | |
| 17576A-G8 | VGB6 | C57BL/6NTac | |
| 17576A-A1 | VGB6 | C57BL/6NTac | |
