| Gene | Bcl6b |
| Chromosome | 11 |
| Deletion Start | 70,229,156 |
| Deletion End | 70,225,927 |
| Deletion Size | 3,230 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,229,109 | 70,229,128 | TGGGCTACGTCCGAGAGTTC |
| TUP | 70,229,086 | 70,229,105 | CGCCACTCCTCCGACGTGCT |
| TUR | 70,229,061 | 70,229,082 | CAGGCGCAGCTCATTCAGATTG |
| TDF | 70,226,170 | 70,226,193 | GAAAGGTGGTTATGGAAAGGTTGG |
| TDP | 70,226,145 | 70,226,168 | ACCTGACAGACCTGTCCTCCCACA |
| TDR | 70,226,096 | 70,226,116 | CAGCCGCAGTTGACTCTTGTG |
PCR Primers
| Primer | Sequence |
| SU | GGGCTGCAGATCATATTTGG |
| SD | CTCAAGCCAAAGGAGAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17577A-A7 | VGB6 | C57BL/6NTac | |
| 17577A-B12 | VGB6 | C57BL/6NTac | |
| 17577A-G4 | VGB6 | C57BL/6NTac | |
| 17577A-B9 | VGB6 | C57BL/6NTac | |
