| Gene | Cab39l |
| Chromosome | 14 |
| Deletion Start | 59,526,809 |
| Deletion End | 59,496,837 |
| Deletion Size | 29,973 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,497,736 | 59,497,756 | CAGCTCGCAAATGTACTGACC |
| TUP | 59,497,759 | 59,497,784 | AAGACTAAGAAGGTGCTGCACACCCG |
| TUR | 59,497,786 | 59,497,807 | CACCATCTCTACTGCTCCACTC |
| TDF | 59,525,910 | 59,525,930 | AGCAGGCTGTGCTCAGATGAC |
| TDP | 59,525,936 | 59,525,962 | AAATGTCACTCGTGTGCTTCTGAGCCC |
| TDR | 59,526,026 | 59,526,047 | GTGGGCTCTAAGGACTGAACTC |
PCR Primers
| Primer | Sequence |
| SU | AACTTGAAACTCAATTTGATCAG |
| SD | AACATACTTCCTTCCAAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 138 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17581A-B10 | VGB6 | C57BL/6NTac | |
| 17581A-E12 | VGB6 | C57BL/6NTac | |
