| Gene | Cbx5 |
| Chromosome | 15 |
| Deletion Start | 103,213,205 |
| Deletion End | 103,199,564 |
| Deletion Size | 13,642 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,212,458 | 103,212,479 | GTTGTAAGCTGCCGTGTGAATG |
| TUP | 103,212,430 | 103,212,456 | TGGAAATCAGACCACAGTTCTCTGGGA |
| TUR | 103,212,404 | 103,212,424 | TCGGTGTTTAAGAGCACTGTC |
| TDF | 103,199,889 | 103,199,908 | CCTCCAGCCCTGTAAAGACC |
| TDP | 103,199,852 | 103,199,879 | TTGAGATCGAACCAGCTAACCTCAGACA |
| TDR | 103,199,817 | 103,199,839 | GGAGACATGAAACTGAGGCAATC |
PCR Primers
| Primer | Sequence |
| SU | TTTGAGTTCCTGGCTCAGAAG |
| SD | AATCTCCCAAAGCAACCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17582A-B11 | VGB6 | C57BL/6NTac | |
| 17582A-H8 | VGB6 | C57BL/6NTac | |
| 17582A-G9 | VGB6 | C57BL/6NTac | |
| 17582A-E11 | VGB6 | C57BL/6NTac | |
| 17582A-F10 | VGB6 | C57BL/6NTac | |
| 17582A-E1 | VGB6 | C57BL/6NTac | |
