| Gene | Ccnl1 |
| Chromosome | 3 |
| Deletion Start | 65,958,125 |
| Deletion End | 65,946,600 |
| Deletion Size | 11,526 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,957,340 | 65,957,363 | CCCGTAGAGCAATGTTTCAAGAAC |
| TUP | 65,957,299 | 65,957,326 | CCCAGTTCACGTGCTCTGTTAACCAAGA |
| TUR | 65,957,244 | 65,957,264 | GGGTCAGAATCAAGCTACCTC |
| TDF | 65,946,734 | 65,946,754 | GCAAGACTCGGGATCACTCAG |
| TDP | 65,946,704 | 65,946,732 | TGTTACCAAGAAACACAGGCACGAAAGGG |
| TDR | 65,946,674 | 65,946,692 | GAGATCGTTCACGCCTGTC |
PCR Primers
| Primer | Sequence |
| SU | CCGGTGACTAAAGCCATTACC |
| SD | CAAGTCTGCAAGTTTCATCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17583A-B1 | VGB6 | C57BL/6NTac | |
| 17583A-B5 | VGB6 | C57BL/6NTac | |
| 17583A-H7 | VGB6 | C57BL/6NTac | |
| 17583A-A7 | VGB6 | C57BL/6NTac | |
