| Gene | Cdc45 |
| Chromosome | 16 |
| Deletion Start | 18,811,562 |
| Deletion End | 18,781,837 |
| Deletion Size | 29,726 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,811,389 | 18,811,408 | AGCGTCCCTGTGTTCAACTC |
| TUP | 18,811,359 | 18,811,385 | TGTCCACGTTTGCTTCTACAGAGGGTC |
| TUR | 18,811,339 | 18,811,358 | TCCGAGGCCACAAAGAGAAG |
| TDF | 18,782,708 | 18,782,727 | GTCTCAGGCAGGCTTCTTTG |
| TDP | 18,782,680 | 18,782,705 | CCAGGCAGCTGTAGCTATGGCATTGT |
| TDR | 18,782,651 | 18,782,669 | GGAGCTGCTGTTCATGGTG |
PCR Primers
| Primer | Sequence |
| SU | TGTCTGCCAAACATAGGTGG |
| SD | TGTGGGAGAATAGTGAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17586A-A1 | VGB6 | C57BL/6NTac | |
| 17586A-F4 | VGB6 | C57BL/6NTac | |
| 17586A-B1 | VGB6 | C57BL/6NTac | |
| 17586A-B10 | VGB6 | C57BL/6NTac | |
| 17586A-E1 | VGB6 | C57BL/6NTac | |
| 17586A-A10 | VGB6 | C57BL/6NTac | |
