| Gene | Cdca3 |
| Chromosome | 6 |
| Deletion Start | 124,833,187 |
| Deletion End | 124,830,534 |
| Deletion Size | 2,654 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,830,569 | 124,830,587 | GCCTCTGCCACGCAACAAG |
| TUP | 124,830,589 | 124,830,614 | AAGTGGCTCGAGTAGCAGACCCTCGT |
| TUR | 124,830,629 | 124,830,649 | TGAATAGGAGTGCGCTGGATG |
| TDF | 124,832,381 | 124,832,398 | AGCTGCCAGCCAGAACTC |
| TDP | 124,832,402 | 124,832,425 | CAAGCCCTCCAGAGACCCAGAGAC |
| TDR | 124,832,459 | 124,832,480 | TCTAGCTTCTCTTCCCTCCATC |
PCR Primers
| Primer | Sequence |
| SU | TTCTCTAGCTGGTGTTCGGG |
| SD | AGGAGATGACCACCTTTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17587A-A10 | VGB6 | C57BL/6NTac | |
| 17587A-B3 | VGB6 | C57BL/6NTac | |
| 17587A-B7 | VGB6 | C57BL/6NTac | |
| 17587A-F4 | VGB6 | C57BL/6NTac | |
| 17587A-E3 | VGB6 | C57BL/6NTac | |
| 17587A-E8 | VGB6 | C57BL/6NTac | |
