17588   Chd4     Status: ES cell colonies screened / QC positives

GeneChd4
Chromosome6
Deletion Start125,130,098
Deletion End125,097,169
Deletion Size32,930
Genome Build38
MGI IDMGI:1344380
NCBI Gene ID107932
BAC ID320F9
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,097,560125,097,582AGGTTCTTGGAGGAATGCAGGTG
TUP125,097,584125,097,607TTCCGACTCTGGTGGTGACATGGT
TUR125,097,621125,097,641AGGGCTCCCGCAACAGAAATC
TDF125,129,696125,129,718CCAGGTAAAGCGAGTGTCAAGAG
TDP125,129,719125,129,744CGTATCCTGTGTATGCCAGCGTGACA
TDR125,129,775125,129,793GCCGTGAGCTGTGGTAGTC

PCR Primers

PrimerSequence
SUTCCCAAGTGACCTTGTGTCC
SDAGAGGTAGCTGTGTCTCGCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR82 bp++-
TDFTDR98 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17588A-B4VGB6C57BL/6NTac
17588A-G10VGB6C57BL/6NTac
17588A-G7VGB6C57BL/6NTac
17588A-F1VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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