| Gene | Clk2 |
| Chromosome | 3 |
| Deletion Start | 89,176,599 |
| Deletion End | 89,167,038 |
| Deletion Size | 9,562 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,167,235 | 89,167,256 | ACGTTACATGATGAGGCCAGTG |
| TUP | 89,167,260 | 89,167,281 | TCAGCAGCTGGTTGAAGGCCAA |
| TUR | 89,167,290 | 89,167,311 | TGTCAGGCACATTCGTCCATAC |
| TDF | 89,176,493 | 89,176,513 | GCCCTTCAGCATCCTTTCTTC |
| TDP | 89,176,515 | 89,176,536 | CCTGCCTTCGGACTGAGCCACC |
| TDR | 89,176,546 | 89,176,563 | CGACTGGAGTCCCACAAC |
PCR Primers
| Primer | Sequence |
| SU | CAGCAGAGATGAACTGTGGC |
| SD | ACCCTCGAACCTCAAATTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17589A-A4 | VGB6 | C57BL/6NTac | |
| 17589A-A10 | VGB6 | C57BL/6NTac | |
| 17589A-A11 | VGB6 | C57BL/6NTac | |
| 17589A-B9 | VGB6 | C57BL/6NTac | |
| 17589A-C2 | VGB6 | C57BL/6NTac | |
| 17589A-C7 | VGB6 | C57BL/6NTac | |
