17597   D1Ertd622e     Status: ES cell colonies screened / QC positives

GeneD1Ertd622e
Chromosome1
Deletion Start97,646,335
Deletion End97,645,683
Deletion Size653
Genome Build38
MGI IDMGI:1277184
NCBI Gene ID52392
BAC ID329D15
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,646,15997,646,181TCCTCCACATGGACTCTAACTAC
TUP97,646,13697,646,158TTGGTTGGCTTCACCACTGGTGA
TUR97,646,10797,646,128TGCACTTCTGGGCTAACTTCAG
TDF97,645,95597,645,972AATGGCAGGAGGCGAAGG
TDP97,645,93297,645,954CGGATGCCCAGCTCAGGAGACAA
TDR97,645,90397,645,926AAGGTTCATAAGGTAAGGGTTTGG

PCR Primers

PrimerSequence
SUGCATACCAAGGAGTAAACTGGC
SDGAGATCCAAGAAGCACTGGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR75 bp++-
TDFTDR70 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17597C-B10VGB6C57BL/6NTac
17597C-C12VGB6C57BL/6NTac
17597C-D4VGB6C57BL/6NTac
17597C-F1VGB6C57BL/6NTac
17597C-F7VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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