| Gene | Dclre1a |
| Chromosome | 19 |
| Deletion Start | 56,547,093 |
| Deletion End | 56,529,447 |
| Deletion Size | 17,647 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,546,739 | 56,546,760 | AAGGTGCGCCCTGTTTATGATG |
| TUP | 56,546,714 | 56,546,736 | TACTGCCCAAGCTGCCAGATGCC |
| TUR | 56,546,688 | 56,546,707 | GCGTCTGACCCAGCAATGAG |
| TDF | 56,530,348 | 56,530,367 | CCCAGTGGCTCTCAGGATTC |
| TDP | 56,530,320 | 56,530,347 | CCAGTGCTTCAGCCTTTAATGTTCACGC |
| TDR | 56,530,287 | 56,530,306 | GCAGCGGAGACAACTTTAGG |
PCR Primers
| Primer | Sequence |
| SU | ACCGTCAGTGCTTGACATTC |
| SD | CCCATCCAAATATGCTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17599A-A2 | VGB6 | C57BL/6NTac | |
| 17599A-A12 | VGB6 | C57BL/6NTac | |
| 17599A-G5 | VGB6 | C57BL/6NTac | |
| 17599A-E7 | VGB6 | C57BL/6NTac | |
| 17599A-E12 | VGB6 | C57BL/6NTac | |
| 17599A-G2 | VGB6 | C57BL/6NTac | |
