| Gene | Dlst |
| Chromosome | 12 |
| Deletion Start | 85,132,754 |
| Deletion End | 85,110,900 |
| Deletion Size | 21,855 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,111,279 | 85,111,296 | TCTTCGCGGTCTCCAAAC |
| TUP | 85,111,297 | 85,111,314 | TAGCGCCTGGCAGGCTCA |
| TUR | 85,111,321 | 85,111,341 | AGTCGGCCAAACTCTGTCATC |
| TDF | 85,132,491 | 85,132,510 | AGGCAGCAGTGGGAGGAAAG |
| TDP | 85,132,515 | 85,132,538 | TGCAACTAGAACCATTGGGTGGGC |
| TDR | 85,132,568 | 85,132,592 | TGTATCCAGCAGATCAGAGACAAAG |
PCR Primers
| Primer | Sequence |
| SU | GGCAGGGAAGGCTTGAAC |
| SD | ACATGGTCCCTGGTGGTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17602A-D8 | VGB6 | C57BL/6NTac | |
| 17602A-E10 | VGB6 | C57BL/6NTac | |
| 17602A-E11 | VGB6 | C57BL/6NTac | |
| 17602A-G12 | VGB6 | C57BL/6NTac | |
| 17602A-G11 | VGB6 | C57BL/6NTac | |
| 17602A-F8 | VGB6 | C57BL/6NTac | |
